Effects of harringtonine on peroxidation and dynamic microstructure of membrane lipids of myocardial sarcoplasmic reticulum in rat

A I M : T o s t u d y t h e d f e c m o f h a r r i n g t o n in e ( H a r ) on pe~xidaifon and dynamic mierostrueture of m ~ l ! b r a ltl e il p i d s a n d th e r e l a t i o n s h i p b e t w e e n ht e m . METHoDS: A nanose~nd time-resolved lfuom. m eter w a8 used to study ht e e fects of I-Iar on dynamic m ierostrueture of sarcoplasmic reticulum (SR)vesicl髑 from ratmyocardium. The~-nount ofilpid pezoxide wa8 measured with thiobarbituric add testand expressed asainountofm alona1.de&ydlc add (MAA ). RESULTS: liar 10 nag ·LI1 i a c c e s s e d M A A i n S R ( fr o m 1 . 7 ± 0 . 8 协 5 . 4 土 5 . 1 ~tmol/gprotein,P<0.05). witl1theincre~eof M A A , ht e m e m b r a n e v i s e o d t y a l s o ni c r e ~ s e d , w h i l e thewobbliz】g angIeof htephospholipid molecul~ decreased(r= 一0.8565,P<0.01).the . 册 a3e activity decreased (r = 一 0.8714。 P < 0 . O 1 ) , ht e lf u o r e s c e n c e ni t e n s i t y a n d t h e fl u o r e s c e n c e l i f e t i m e d e c r e a s e d . C o N C L U S I o N : l i a r m a y c a I l s e th e p e a x ~ x i d a t i o n o f m e m b r a n e l i p i d s in SR, which afected the mobilty of hte p h o s p h o li p i d b i l a y e r s a n d t h e f u n c ti o n o f m e m b r a n e proteins.