Effects of dexamethasone, ibuprofen, and ligustrazini on lipopolysaccharides-induced tumor necrosis factor alpha production
Abstract
AIM: To study the influence of dexamethasone (Dex), ibuprofen (Ibu), and
ligustrazini (Lig) on lipopolysaccharides (LPS)-induced tumor necrosis factor
alpha (TNF alpha) gene expression (both mRNA and protein).
METHODS: TNF alpha in supernatants of human whole blood was measured by ELISA;
The TNF alpha mRNA was assessed by slot blot analysis.
RESULTS: LPS-induced TNF alpha production was in a dose-dependent manner. TNF
alpha levels in the whole blood increased markedly at 3 h and peaked at 6 h. The
induction of TNF alpha mRNA was very rapid, peaking at 2 h after LPS challenge.
Dex exerted inhibitory effects on TNF alpha production in a dose-dependent
manner. Ibu and Lig had 2-phase effects on TNF alpha release.
CONCLUSION: Dex, Ibu, and Lig affected TNF alpha gene expression, so they may be
new approaches of anti-TNF alpha for treatment of sepsis.
Keywords:
ligustrazini (Lig) on lipopolysaccharides (LPS)-induced tumor necrosis factor
alpha (TNF alpha) gene expression (both mRNA and protein).
METHODS: TNF alpha in supernatants of human whole blood was measured by ELISA;
The TNF alpha mRNA was assessed by slot blot analysis.
RESULTS: LPS-induced TNF alpha production was in a dose-dependent manner. TNF
alpha levels in the whole blood increased markedly at 3 h and peaked at 6 h. The
induction of TNF alpha mRNA was very rapid, peaking at 2 h after LPS challenge.
Dex exerted inhibitory effects on TNF alpha production in a dose-dependent
manner. Ibu and Lig had 2-phase effects on TNF alpha release.
CONCLUSION: Dex, Ibu, and Lig affected TNF alpha gene expression, so they may be
new approaches of anti-TNF alpha for treatment of sepsis.