Suppressive effects of adenosine on nonspecific and humoral immunities in mice

Adenosine (Ade) 1.3, 13, 130 mg.kg-1 ip inhibited the ability of peripheral leukocytes and peritoneal macrophages in phagocytosing the Staphylococcus albus with [3H]TdR incorporation in mice, declined the hemolytic ability of plaque-forming cells and the production of antibody in mice immunized by sheep erythrocytes. Ade 13, 130 mg.kg-1 ip decreased the mouse serum muramidase (lysozyme) concentration. Dipyridamole (Dip) 10 mg.kg-1 ip attenuated the effects of Ade 130 mg.kg-1 on humoral immunity reaction, but the nonspecific immunity was not attenuated. These results showed that the uptake of Ade may play an important role in the effects of Ade on humoral immunity reaction. Aminophylline (Ami) 100 mg.kg-1 ip attenuated the effects of Ade 130 mg.kg-1 on hemolytic ability of plaque-forming cells and the ability of peripheral leukocytes in phagocytosing Staphylococcus albus. These results suggested that the effects of Ade on murine humoral and nonspecific immunity reaction were mediated by Ade A2 receptor (A2DR).