Original Article

Antitumor effects of new-type recombinant interleukin-2

Xue-Sheng Feng, Xiu-Juan Zheng, Qiu-Sheng Si, Yun-Lu Lin, Yuan Chang, Pei-Fang Fan, Jian-Liang Yu, Shu-Ren Zhang, Xin-Yuan Liu

Abstract

Two new human recombinant interleukin-2 (rIL-2), 125-Ser-rIL-2, and 125-Ala-rIL-2, were generated by protein engineering technique. Both of them maintained the proliferation of natural killer (NK) cells, CTLL-2 cells and their long-term propagations. The mutated new rIL-2 also enhanced the bioactivity of NK cells and the cytotoxicity of tumor-infiltrating lymphocytes (TIL) against the target tumor cells. The above results were all compared with that of the native rIL-2 and a similarity between them was found, which indicates that new type rIL-2 could be used for adoptive immunotherapy of malignant diseases.
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