Influence of the 2nd and 3rd grade abstracts from scorpion venom of Buthus martensii Karsch on action potentials of fast response myocardiocytes
Abstract
AIM: To screen the active components of sodium channel blockade action from Buthus martensii Karsch (BMK) scorpion venom.
METHODS: Myocardiocytes of mice were cultured. Action potentials of fast response myocardiocytes were recorded. The effects of 22 contents of BMK scorpion venom in a concentration of 3 mg.L-1 were tested and compared with that of TTX 2.5 mg.L-1, nimodipine (Nim) 3 mg.L-1 and BaCl2 24.4 mg.L-1.
RESULTS: BMK-1 and other 19 contents significantly decreased depolarization parameters Vmax, APA, OS, and MDP, which was similar to that of TTX and different from that of Nim and BaCl2.
CONCLUSION: The variations in the action potentials of fast response myocardiolcytes indicated that 20 contents of BMK scorpion venom had sodium channel blockade action.
Keywords:
METHODS: Myocardiocytes of mice were cultured. Action potentials of fast response myocardiocytes were recorded. The effects of 22 contents of BMK scorpion venom in a concentration of 3 mg.L-1 were tested and compared with that of TTX 2.5 mg.L-1, nimodipine (Nim) 3 mg.L-1 and BaCl2 24.4 mg.L-1.
RESULTS: BMK-1 and other 19 contents significantly decreased depolarization parameters Vmax, APA, OS, and MDP, which was similar to that of TTX and different from that of Nim and BaCl2.
CONCLUSION: The variations in the action potentials of fast response myocardiolcytes indicated that 20 contents of BMK scorpion venom had sodium channel blockade action.