Binding sites of ZNC(C)PR, a pentapeptide fragment of argipressin, in rat brain
Abstract
AIM: To study the binding sites of ZNC (C) PR, a C-terminal pentapeptide fragment of argipressin, in rat brain.
METHODS: Using radioligand assay and radioligand [35S]ZNC(C)PR.
RESULTS: It was found that there exist native binding sites bound ZNC(C)PR with high affinity in a saturable, reversible and specific manner. Scatchard and kinetic analyses showed that these sites were homogeneous with a Kd value of 1.69 +/- 0.16 nmol.L-1 (in the presence of MgCl2 10 mmol.L-1). The binding of ZNC(C)PR to the sites was at a higher level in the brain regions (such as amygdala, cortex,) and was affected by Mg2+.
CONCLUSION: These binding sites represented a new type of receptors and mediated the action of ZNC(C)PR on memory.
Keywords:
METHODS: Using radioligand assay and radioligand [35S]ZNC(C)PR.
RESULTS: It was found that there exist native binding sites bound ZNC(C)PR with high affinity in a saturable, reversible and specific manner. Scatchard and kinetic analyses showed that these sites were homogeneous with a Kd value of 1.69 +/- 0.16 nmol.L-1 (in the presence of MgCl2 10 mmol.L-1). The binding of ZNC(C)PR to the sites was at a higher level in the brain regions (such as amygdala, cortex,) and was affected by Mg2+.
CONCLUSION: These binding sites represented a new type of receptors and mediated the action of ZNC(C)PR on memory.