Ultrastructural study on effect of primaquine on sporogonic stage of Plasmodium yoelii nigeriensis

Ultrastructural changes of oocysts and sporozoites of Plasmodium yoelii nigeriensis was observed. Anopheles stephensi were allowed to obtain blood meal from the mice which had been administered with primaquine diphosphate at different doses and times. Mosquitoes were directed and prepared 6-13 d following infection. Electron microscopy showed that the development and morphology of a number of oocysts and sporozoites in infected mosquitoes after treatment became abnormal. The cytoplasma of the oocysts were partially or totally dissolved and formed vacuoles. The walls of oocysts were thicken. The nuclei and organelles of oocysts and sporozoites were destroyed or damaged. The nuclei and organelles of oocysts and sporozoites were destroyed or damaged. The extent of damage of the oocysts and sporozoites related to the dose of primaquine and the time after the drug administration.