Establishment of human lung adenocarcinoma model in nude mice and sensitivity of the transplanted tumor to antitumor drugs
Abstract
A transplanted human adenocarcinoma, coded LAX-83, was successfully transplanted from surgical specimen of a lung adenocarcinoma patient, and had maintained for 2 yr through 57 passages in the nude mice. LAX-83 revealed to possess fast growth, short latent period of 3-5 d, 100% success rate in each passage and no spontaneous regression of the tumor during the surviving time of the nude mice. The interval between two passages was about 2 wk. 3 wk after the inoculation the average weight of the tumor was 3.1±1.3 g. The tumor growing in nude mice was confirmed to acquire human characteristics by chromosome analysis, isoenzyme studies and morphologic investigations.
Seven antitumor drugs, which have been used in the treatment of human lung adenocarcinoma, were tested in LAX-83-nude mice system during 13-19 th passage. From the d 6 after inoculation the drug were given ip to the nude mice daily for 10 d. 5 d after the cessation of the treatment all treated mice were killed and dissected the tumors. The therapeutic effect was evaluated by the inhibition rate of the tumor, cyclophosphamide, vincristine, adriamycin, mitomycin C, 5-Fu and cis-DDP were effective against LAX-83. while methotrexate was not. The effects of the antitumor drugs on LAX-83 exhibited a good correlation to the clinical therapeutic effects on lung adenocarcinoma and suggested that LAX-83-nude mice system is an appropriate model for the studies on antitumor drugs.
Keywords:
Seven antitumor drugs, which have been used in the treatment of human lung adenocarcinoma, were tested in LAX-83-nude mice system during 13-19 th passage. From the d 6 after inoculation the drug were given ip to the nude mice daily for 10 d. 5 d after the cessation of the treatment all treated mice were killed and dissected the tumors. The therapeutic effect was evaluated by the inhibition rate of the tumor, cyclophosphamide, vincristine, adriamycin, mitomycin C, 5-Fu and cis-DDP were effective against LAX-83. while methotrexate was not. The effects of the antitumor drugs on LAX-83 exhibited a good correlation to the clinical therapeutic effects on lung adenocarcinoma and suggested that LAX-83-nude mice system is an appropriate model for the studies on antitumor drugs.