Micronucleus formation in mouse polychromatic erythrocyte induced by platinum coordination complexes
Abstract
The genotoxicities of cisplatin,, malonato-1,2-diaminocyclohexane-platinum (II) (PHM), and sulfato-1,2-diaminocyclohexaneplatinum (II) (SHP) were studied by bone marrow polychromatic erythrocyte (PCE) micronucleus assay of male mice. An increase in the number of micronucleated PCEs was seen at and over the ip dose of cisplatin 0.19 mg/kg, PHM 5.6 mg/kg, and SHP 0.18 mg/kg. The dose-effect curves for these chemicals showed 2 or 3 phases with different slopes.
A significant increase of micronucleated PCEs did not occur until 18 h after cisplatin. For cisplatin, the maximum of micronucleated PCEs was dose-dependent; the frequency of micronuclei reached the highest levels at 24 h after 0.24 mg/kg and at 30 h after 1.5 mg/kg. The results suggest that the expression of micronuclei induced by cisplatin may be dependent upon progression of cells through the DNA synthesis of the cell cycle, and the mitotic delay was induced. When genotoxicities of platinum coordination complexes were evaluated by the bone marrow PCE micronucleus assay, mice should be sampled at 24 or 30 h after a single ip.
Keywords:
A significant increase of micronucleated PCEs did not occur until 18 h after cisplatin. For cisplatin, the maximum of micronucleated PCEs was dose-dependent; the frequency of micronuclei reached the highest levels at 24 h after 0.24 mg/kg and at 30 h after 1.5 mg/kg. The results suggest that the expression of micronuclei induced by cisplatin may be dependent upon progression of cells through the DNA synthesis of the cell cycle, and the mitotic delay was induced. When genotoxicities of platinum coordination complexes were evaluated by the bone marrow PCE micronucleus assay, mice should be sampled at 24 or 30 h after a single ip.