Effects of oridonin on DNA, RNA and protein syntheses of leukemia L 1210 cells studied by autoradiography
Abstract
The incorporation of [3H]TdR into DNA of L 1210 cells was inhibited by oridonin in vivo. The labelling index and the mean grain count decreased by 56 and 55%, respectively.
The incorporation of [3H]UR into RNA of L 1210 cells was inhibited by oridonin in mice. The labelling index and the mean grain count were lowered to 32 and 47%, respectively, of the controlled value in 9 h.
The incorporation of [3H]Leu was also inhibited by oridonin. The labelling index and the mean grain count fell to 55 and 44%, respectively, of the controlled value in mice.
These results indicate that oridonin can inhibit DNA, RNA and protein syntheses, and the inhibition of DNA syntheses was more prominent and earlier than that of RNA and protein syntheses.
Keywords:
The incorporation of [3H]UR into RNA of L 1210 cells was inhibited by oridonin in mice. The labelling index and the mean grain count were lowered to 32 and 47%, respectively, of the controlled value in 9 h.
The incorporation of [3H]Leu was also inhibited by oridonin. The labelling index and the mean grain count fell to 55 and 44%, respectively, of the controlled value in mice.
These results indicate that oridonin can inhibit DNA, RNA and protein syntheses, and the inhibition of DNA syntheses was more prominent and earlier than that of RNA and protein syntheses.