Original Articles

Effects of anemodeanin A on DNA, RNA and protein of tumor cells in vitro and plasma cAMP in mice

Li-sheng Liu, Xian-hua Xiao, Long-di Zhang, Rong-liang Zheng, Feng-e Wu, Zi-qin Zhu

Abstract

Oleanolic acid 3-O-alpha-L-rhamnopyranosyl-(1-2)-beta-D-glucopyranosyl-(1-2)-alpha-L-arabinopyranoside(anemodeanin A) was extracted from Anemone raddeana Regel. Anemodeanin A 30 microg/mL obviously inhibited DNA, RNA and protein syntheses of sarcoma 180 and ascetic hepatoma cells during the period of 12-48 h after exposure in vitro. ID50 of anemodeanin A on DNA synthesis in ascetic hepatoma cells at 48 h was 21 microg/mL. This drug ip 10 mg/kg* 5 d elevated the plasma cAMP of mice by 61%.
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