Radioimmunoassay for paraoxon
Abstract
Having effectively inhibited serum esterases for preventing enzymic hydrolysis of paraoxon during assay, the sensitivity of RIA for paraoxon was 0.20 ng (0.72 pmol). Untreated blood sample could be assayged directly. Anti-paraoxon antiserum was very specific with the affinity constant of 2.13*10(8) M(-1) and working titre of 1:1000. At 1-2, 10 and 23.5 degrees, the rate constants for dissociation of [3H]paraoxon-antibody complex were 4.70*10(-3), 1.55*10(-2) ad 2.38*10(-1) min (-1), respectively.
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