Effects of bimolane on size, metastases and cyclic AMP levels of Lewis lung carcinoma

Bimolane (AT1727), 1,2-bis (4-morphplinomethy1-3,5-dioxopiperazinyl)-ethane, is a derivative of ICRF154. It was first synthesized by Shanghai Institute of Materia Medica, Chinese Academy of Sciences. It slightly inhibited tumor growth and markedly inhibited the spontaneous pulmonary metastases of Lewis lung carcinoma in C57BL/6 mice. Adenosine cyclic-3’, 5’-monophosphate (cAMP) was assayed by Camp-dependent protein kinase binding assay in tumor tissue and plasma. Bimolane elevated moderately the Camp levels in primary tumor tissue of Lewis lung carcinoma. The cAMP level of plasma in normal C57BL/6 mice was not significantly different from that in C57BL/6 mice bearing Lewis lung carcinoma. Bimolane did not change the cAMP levels of plasma in C57BL/6 mice bearing Lewis ling carcinoma.