Knockdown of Stat3 expression using RNAi inhibits growth of laryngeal tumors in vivo
Abstract
Aim: To study the effect of pSilencer 1.0-U6-siRNA-stat3 on the growth of human laryngeal tumors in nude mice.
Methods: Hep2 cells were transplanted into nude mice, then at the time of tumor formation, growth rates were observed. After the tumor formed, pSilencer 1.0-U6-siRNA- stat3 was injected. Tumor volumes were calculated, and growth curves were plotted. Representative histological sections were taken from mice bearing transplantation tumors in both treated and control groups, and start3 , pTyr-stat3 , Bcl-2 , cyclin D1 , and surviving expression were detected by Western blotting. survivin mRNA levels were detected by Northern blotting, hematoxylin and eosin staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) assay to confirm the apoptosis of tumors.
Results: In nude mice, pSilencer 1.0-U6-siRNA-stat3 significantly suppressed the growth of tumors compared with controls (P <0.01). In suppressed stat3 expression, and downregulated BcL2 , cyclin D1 , and surviving expression within the tumor. This significantly induced apoptosis of the tumors.
Conclusion: pSilencer 1.0-U6-siRNA- stat3 was able to inhibit the growth of transplanted human laryngeal tumors in nude mice and induce apoptosis.
Keywords:
Methods: Hep2 cells were transplanted into nude mice, then at the time of tumor formation, growth rates were observed. After the tumor formed, pSilencer 1.0-U6-siRNA- stat3 was injected. Tumor volumes were calculated, and growth curves were plotted. Representative histological sections were taken from mice bearing transplantation tumors in both treated and control groups, and start3 , pTyr-stat3 , Bcl-2 , cyclin D1 , and surviving expression were detected by Western blotting. survivin mRNA levels were detected by Northern blotting, hematoxylin and eosin staining and terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) assay to confirm the apoptosis of tumors.
Results: In nude mice, pSilencer 1.0-U6-siRNA-stat3 significantly suppressed the growth of tumors compared with controls (P <0.01). In suppressed stat3 expression, and downregulated BcL2 , cyclin D1 , and surviving expression within the tumor. This significantly induced apoptosis of the tumors.
Conclusion: pSilencer 1.0-U6-siRNA- stat3 was able to inhibit the growth of transplanted human laryngeal tumors in nude mice and induce apoptosis.