Article

Cardioprotective mechanisms of salvianic acid A sodium in rats with myocardial infarction based on proteome and transcriptome analysis

Dan Jia1, Cheng-zhong Zhang1, Yan Qiu2, Xiao-fei Chen1, Lin Jia3, Alex F. Chen1, Yi-feng Chai1, Zhen-yu Zhu1, Jin Huang4, Chuan Zhang1,5
1 School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2 Department of Pharmacy, Pudong New Area People’s Hospital Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201200, China
3 Department of Endodontics, Panyu Stomatological Hospital (Guangdong Provincial Stomatological Hospital) Affiliated to Southern Medical University, Guangzhou 511400, China
4 Department of Pharmacy, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China
5 School of Medicine, Shanghai University, Shanghai 200444, China
Correspondence to: Jin Huang: john70550@aliyun.com, Chuan Zhang: zhangchuan@smmu.edu.cn,
DOI: 10.1038/s41401-019-0265-1
Received: 21 February 2019
Accepted: 27 May 2019
Advance online: 28 June 2019

Abstract

Ischemic heart diseases (IHDs) cause great morbidity and mortality worldwide, necessitating effective treatment. Salvianic acid A sodium (SAAS) is an active compound derived from the well-known herbal medicine Danshen, which has been widely used for clinical treatment of cardiovascular diseases in China. This study aimed to confirm the cardioprotective effects of SAAS in rats with myocardial infarction and to investigate the underlying molecular mechanisms based on proteome and transcriptome profiling of myocardial tissue. The results showed that SAAS effectively protected against myocardial injury and improved cardiac function. The differentially expressed proteins and genes included important structural molecules, receptors, transcription factors, and cofactors. Functional enrichment analysis indicated that SAAS participated in the regulation of actin cytoskeleton, phagosome, focal adhesion, tight junction, apoptosis, MAPK signaling, and Wnt signaling pathways, which are closely related to cardiovascular diseases. SAAS may exert its cardioprotective effect by targeting multiple pathways at both the proteome and transcriptome levels. This study has provided not only new insights into the pathogenesis of myocardial infarction but also a road map of the cardioprotective molecular mechanisms of SAAS, which may provide pharmacological evidence to aid in its clinical application.
Keywords: Traditional Chinese medicine; salvianic acid A sodium; myocardial infarction; cardioprotective; proteome; transcriptome

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